|
KMID : 0603520030080030151
|
|
Journal of Korean Association of Cancer Prevention
2003 Volume.8 No. 3 p.151 ~ p.159
|
|
|
Effect of Conjugated Linoleic Acid and Its Isomers on the Apoptosis of Caco-2 Colon Cancer Cells
|
|
Kim Eun-Ji
YoonPark Jung-Han
|
|
|
Abstract
|
|
|
|
Conjugated linoleic acid (CLA) is a group of positional and geometric isomers of linoleic acid (LA) and has chemoprotective properties in a variety of experimental cancer models. Cis-9,trans-11 and trans-10,cis-12 CLA are the principal isomers found in foods. The present study was performed to determine whether a mixture of CLA isomers and the two individual CIA isomers induce apoptotic cell death in Caco-2 cells. Caco-2 cells were incubated in serum-free medium containing 100 pM of the mixture of CLA isomers, 100 ,aM linoleic acid, 10 uM cis-9,trans-1 1 CLA, or 10 pM trans-10,cis-12 CLA. The mixture of CLA isomers and trans-10,cis-12 CLA inhibited Caco-2 cell growth. By contrast cis-9,trans-11 CLA had no effect. Cells treated with the mixture of CLA isomers and trans-10,cis-12 CLA produced a distinct oligonucleosomal ladder with different sizes of DNA fragments, a typical characteristic of cells undergoing apoptosis and were stained with Annexin-V. The mixture of CLA isomers and trans-10,cis-12 CLA increased the activity of caspase-3, which is a central regulator of apoptosis. These results suggested that the growth inhibitory effect of the mixture of CLA isomers may, at least in part, be attributed to caspase-3 dependent apoptosis in Caco-2 cells.
|
|
|
KEYWORD
|
|
|
Conjugated linoleic acid(CLA), trans-10, cis-12 CLA, Apoptosis, Caspase-3
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|
|